Technology
We develop ultrasensitive sequencing technologies that are easy to use and can be performed in any molecular biology lab. Sequencing is used to read the DNA code and ultrasensitive sequencing is necessary to analyze rare mutations or clones. Our technology has a wide range of applications. It is specifically adapted to DNA difficult to analyze.
Ultrasensitive targeted sequencing
Our technology is based on next-generation sequencing (NGS) and utilizes unique molecular barcodes to reduce errors. DNA oligos, called primers, are used in panels to target genes of interest. Our proprietary design minimizes the need for intermediate cleaning steps, enabling a fast and easy protocol. The final sequencing library can be sequenced on any Illumina® sequencer.
Feature highlights
High sensitivity
Easy to use protocol
Customizable panels
Rapid and scalable protocol
Our technology features a simple two-step protocol that can be completed in three hours with minimal hands-on time and performed in any standard molecular laboratory lab. The proprietary primer design minimizes the need for intermediate cleaning steps, thus enabling a fast and easy library construction protocol.
Error correction with molecular barcoding
Molecular barcodes attached to the DNA make it possible to remove errors occurring during the library construction and sequencing. The process reduces the number of errors by up to one thousandfold compared to conventional sequencing, enabling detection of extremely rare variants even in the presence of a large background. In addition to removing errors, molecular barcoding allows the user to count the number of analyzed molecules which is important in applications such as circulation tumor DNA analysis and immune repertoire sequencing.